What Are The Core Benefits And Application Areas Of Neutral Protease?

 

Neutral proteases are metalloproteinases that function under neutral pH conditions (optimal pH 6.0–8.0) and are primarily derived from Bacillus polymyxa.

 

Their core active site consists of zinc ions and a conserved amino acid sequence (histidine-glutamic acid-histidine). They specifically hydrolyze fibronectin, laminin, and type IV collagen in the extracellular matrix, but exhibit low activity in degrading fibrous collagen.

 

 

1. Mildness and Efficiency

• It operates efficiently at physiological pH (7.0–8.0) and temperature (37°C), avoiding cell damage from strong acid/alkali environments.
• It precisely degrades cell-matrix junction proteins, preserving cell surface receptors and intercellular junction structures, significantly improving cell activity and functional integrity.

 

2. High Stability

Neutral protease enzyme is insensitive to serum components, and its stability can be enhanced by adding Ca²⁺; chelating agents such as EDTA can rapidly terminate enzyme activity, facilitating reaction control.

 

3. Good Safety

Produced by bacterial fermentation, it carries no risk of mycoplasma or animal virus contamination, making it suitable for clinical-grade cell preparation.

 

 

1. Life Sciences and Medical Research

• Primary Cell Isolation: Isolation of neural stem cells, extraction of epithelial cells
• Organoids and Cell Passaging
• Tissue Engineering: Isolation of intact epidermal and dermal layers, or release of epithelial lamellae from culture media for skin regeneration research.

 

2. Food Industry

• Baking Industry: Hydrolyzes dough proteins, reduces gluten strength, improves biscuit plasticity and texture, and promotes Maillard reactions to enhance flavor.
• Beer Clarification: Degrades cloudy proteins; when combined with other enzymes, it can remove bitterness.
• Protein Hydrolysate Production: Prepares hydrolyzed animal and plant proteins for use in flavorings and nutritional fortifiers.

 

3. Feed Additives

Neutral protease powder can improve the utilization rate of aquatic feed, promote nutrient metabolism and weight gain in fish.

 

 

Fruiterco is an export company that mainly produces plant extracts, health product raw materials, and cosmetic raw materials. The company has a 20+ professional service after-sales team.

 

Our products are sold to North America, Europe, Australia, South America and Asia. Our products have passed quality inspection and also support customer re-inspection. If you would like to buy neutral protease, please contact us: Email: info@fruiterco.com.

 

 

1. Albillos S. M., Busto M. D., Perez-Mateos M., Ortega N. (2007). Analysis by capillary electrophoresis of the proteolytic activity of a Bacillus subtilis neutral protease on bovine caseins. Int. Dairy J. 17, 1195–1200.

2. Belmessikh A., Boukhalfa H., Mechakra-Maza A., Gheribi-Aoulmi Z., Amrane A. (2013). Statistical optimization of culture medium for neutral protease production by Aspergillus oryzae. Comparative study between solid and submerged fermentations on tomato pomace. J. Taiwan. Inst. Chem. E 44, 377–385.

3. Campbell J. M., Peberdy J. F. (1983). Properties of Aspergillus nidulans neutral protease. FEMS Microbiol. Lett. 16, 49–53.

4. Eijsink V. G. H., van der Zee R., van den Burg B., Vriend G., Venema G. (1991). Improving the thermostability of the neutral protease of Bacillus stearothermophilus by replacing a buried asparagine by leucine. FEBS Lett. 282, 13–16.

5. Ma X., Liu Y., Li Q., Liu L., Yi L., Ma L., et al. (2016). Expression, purification and identification of a thermolysin-like protease, neutral protease I, from Aspergillus oryzae with the Pichia pastoris expression system. Protein Expr. Purif. 128, 52–59.

6. https://draxe.com/nutrition/protease/#:~:text=Protease%20enzymes%20increase%20the%20potency,according%20to%20in%20vitro%20research.

7. Mouse islet of langerhans isolation using a combination of purified collagenase and neutral protease

8. Purification and characterization of neutral protease from Aspergillus oryzae Y1 isolated from naturally fermented broad beans